Datasets:
image image | Plate_Well string | Plate string | Well Number int64 | Well string | Characteristics [Organism] string | Term Source 1 REF string | Term Source 1 Accession string | Characteristics [Cell Line] string | Term Source 2 REF string | Term Source 2 Accession string | siRNA Pool Identifier string | siRNA Identifier string | Original Gene Target string | Original RefSeq Target string | Original LocusLink Target float64 | Reagent Design Gene Annotation Build float64 | Gene Identifier string | Gene Symbol string | RefSeq Accession string | RefSeq Hits string | Gene Annotation Comments string | Analysis Gene Annotation Build string | Control Type string | Control Comments string | Channels string | n float64 | ext float64 | ecc float64 | Ato float64 | Nex float64 | Nin float64 | Nto float64 | AF float64 | BC float64 | C float64 | M float64 | LA float64 | P float64 | Has Phenotype string | Phenotype Annotation Level string | Phenotype 1 string | Phenotype 1 Term Name a string | Phenotype 1 Term Accession a string | Phenotype 1 Term Name b string | Phenotype 1 Term Accession b string | Phenotype 2 string | Phenotype 2 Term Name string | Phenotype 2 Term Accession string | Phenotype 3 string | Phenotype 3 Term Name string | Phenotype 3 Term Accession string | Phenotype 4 string | Phenotype 4 Term Name string | Phenotype 4 Term Accession string | Phenotype 5 string | Phenotype 5 Term Name string | Phenotype 5 Term Accession string | Phenotype 6 string | Phenotype 6 Term Name a string | Phenotype 6 Term Accession a string | Phenotype 6 Term Name b string | Phenotype 6 Term Accession b string | Phenotype 7 string | Phenotype 7 Term Name a string | Phenotype 7 Term Accession a string | Phenotype 7 Term Name b string | Phenotype 7 Term Accession b string | Phenotype 8 string | Phenotype 8 Term Name string | Phenotype 8 Term Accession string | Phenotype 9 string | Phenotype 9 Term Name string | Phenotype 9 Term Accession string | Phenotype 10 string | Phenotype 10 Term Name string | Phenotype 10 Term Accession string | Phenotype 11 string | Phenotype 11 Term Name string | Phenotype 11 Term Accession string | Phenotype 12 string | Phenotype 12 Term Name string | Phenotype 12 Term Accession string | Phenotype 13 string | Phenotype 13 Term Name string | Phenotype 13 Term Accession string | Phenotype 14 string | Phenotype 14 Term Name string | Phenotype 14 Term Accession string | Phenotype 15 string | Phenotype 15 Term Name string | Phenotype 15 Term Accession string | Phenotype 16 string | Phenotype 16 Term Name a string | Phenotype 16 Term Accession a string | Phenotype 16 Term Name b string | Phenotype 16 Term Accession b string | Phenotype 17 string | Phenotype 17 Term Name string | Phenotype 17 Term Accession string | Phenotype 18 string |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
HT65_J9 | HT65 | 225 | J9 | Homo sapiens | NCBITaxon | NCBITaxon_9606 | HeLa | EFO | EFO_0001185 | M-032424-00 | D-032424-01;D-032424-02;D-032424-03;D-032424-04 | OR1L3 | NM_001005234 | 52,546,684 | null | 26735 | OR1L3 | NM_001005234 | 4 | null | NCBI36.3, RefSeq release 27, Jan 2008 | null | null | Alexa 488:tubulin;Hoechst:DNA;Tritc:actin | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | |
HT51_E14 | HT51 | 110 | E14 | Homo sapiens | NCBITaxon | NCBITaxon_9606 | HeLa | EFO | EFO_0001185 | M-017807-00 | D-017807-01;D-017807-02;D-017807-03;D-017807-04 | LOC167127 | NM_174914 | 31,341,368 | null | 167127 | UGT3A2 | NM_174914 | 4 | null | NCBI36.3, RefSeq release 27, Jan 2008 | null | null | Alexa 488:tubulin;Hoechst:DNA;Tritc:actin | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | |
HT39_H6 | HT39 | 174 | H6 | Homo sapiens | NCBITaxon | NCBITaxon_9606 | HeLa | EFO | EFO_0001185 | M-010730-00 | D-010730-01;D-010730-02;D-010730-03;D-010730-04 | C6ORF79 | NM_022102 | 13,430,855 | null | 63933 | CCDC90A | NM_001031713 | 4 | null | NCBI36.3, RefSeq release 27, Jan 2008 | null | null | Alexa 488:tubulin;Hoechst:DNA;Tritc:actin | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | |
HT58_E5 | HT58 | 101 | E5 | Homo sapiens | NCBITaxon | NCBITaxon_9606 | HeLa | EFO | EFO_0001185 | M-022958-00 | D-022958-01;D-022958-02;D-022958-03;D-022958-04 | LOC284120 | XM_211339 | 42,661,054 | null | null | null | XM_001721080;XM_211339 | 4;4 | siRNA matched to more than one gene (LOC284120;284120,LOC284120;LOC284120) | NCBI36.3, RefSeq release 27, Jan 2008 | null | null | Alexa 488:tubulin;Hoechst:DNA;Tritc:actin | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | |
HT33_E23 | HT33 | 119 | E23 | Homo sapiens | NCBITaxon | NCBITaxon_9606 | HeLa | EFO | EFO_0001185 | M-019998-00 | D-019998-01;D-019998-02;D-019998-03;D-019998-04 | CHORDC1 | NM_012124 | 6,912,303 | null | 26973 | CHORDC1 | NM_012124 | 4 | null | NCBI36.3, RefSeq release 27, Jan 2008 | null | null | Alexa 488:tubulin;Hoechst:DNA;Tritc:actin | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | |
HT58_K6 | HT58 | 246 | K6 | Homo sapiens | NCBITaxon | NCBITaxon_9606 | HeLa | EFO | EFO_0001185 | M-023762-00 | D-023762-01;D-023762-02;D-023762-03;D-023762-04 | LOC131149 | XM_067228 | 37,550,693 | null | 131149 | LOC131149 | NM_001080440 | 4 | null | NCBI36.3, RefSeq release 27, Jan 2008 | null | null | Alexa 488:tubulin;Hoechst:DNA;Tritc:actin | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | |
HT65_A8 | HT65 | 8 | A8 | Homo sapiens | NCBITaxon | NCBITaxon_9606 | HeLa | EFO | EFO_0001185 | M-032300-00 | D-032300-01;D-032300-02;D-032300-03;D-032300-04 | SPINK5L2 | NM_001001325 | 47,679,098 | null | 408187 | SPINK5L2 | NM_001001325 | 4 | null | NCBI36.3, RefSeq release 27, Jan 2008 | null | null | Alexa 488:tubulin;Hoechst:DNA;Tritc:actin | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | |
HT58_L21 | HT58 | 285 | L21 | Homo sapiens | NCBITaxon | NCBITaxon_9606 | HeLa | EFO | EFO_0001185 | M-024295-00 | D-024295-01;D-024295-02;D-024295-03;D-024295-04 | LOC343702 | XM_293160 | 41,151,301 | null | 343702 | XKR7 | NM_001011718 | 2 | null | NCBI36.3, RefSeq release 27, Jan 2008 | null | null | Alexa 488:tubulin;Hoechst:DNA;Tritc:actin | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | |
HT15_O6 | HT15 | 342 | O6 | Homo sapiens | NCBITaxon | NCBITaxon_9606 | HeLa | EFO | EFO_0001185 | M-011130-00 | D-011130-01;D-011130-02;D-011130-03;D-011130-04 | TNFSF6 | NM_000639 | 4,557,328 | null | 356 | FASLG | NM_000639 | 4 | null | NCBI36.3, RefSeq release 27, Jan 2008 | null | null | Alexa 488:tubulin;Hoechst:DNA;Tritc:actin | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | |
HT31_B22 | HT31 | 46 | B22 | Homo sapiens | NCBITaxon | NCBITaxon_9606 | HeLa | EFO | EFO_0001185 | M-024272-00 | D-024272-01;D-024272-02;D-024272-03;D-024272-04 | KIAA0947 | XM_029101 | 42,657,171 | null | 23379 | KIAA0947 | NM_015325 | 4 | null | NCBI36.3, RefSeq release 27, Jan 2008 | null | null | Alexa 488:tubulin;Hoechst:DNA;Tritc:actin | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | |
HT11_J9 | HT11 | 225 | J9 | Homo sapiens | NCBITaxon | NCBITaxon_9606 | HeLa | EFO | EFO_0001185 | M-012299-00 | D-012299-01;D-012299-02;D-012299-03;D-012299-04 | RUVBL2 | NM_006666 | 5,730,022 | null | 10856 | RUVBL2 | NM_006666 | 4 | null | NCBI36.3, RefSeq release 27, Jan 2008 | null | null | Alexa 488:tubulin;Hoechst:DNA;Tritc:actin | 0 | 0.999 | 0 | 0 | 0.978 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | yes | multiple reagents | BL phenotype (large cells and bright nuclei) | increased cell size phenotype | CMPO_0000128 | bright nuclei phenotype | CMPO_0000154 | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | |
HT65_C7 | HT65 | 55 | C7 | Homo sapiens | NCBITaxon | NCBITaxon_9606 | HeLa | EFO | EFO_0001185 | M-031566-00 | D-031566-01;D-031566-02;D-031566-03;D-031566-04 | MCART6 | XM_377034 | 42,662,602 | null | 401612 | MCART6 | NM_001012755 | 4 | null | NCBI36.3, RefSeq release 27, Jan 2008 | null | null | Alexa 488:tubulin;Hoechst:DNA;Tritc:actin | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | |
HT34_F7 | HT34 | 127 | F7 | Homo sapiens | NCBITaxon | NCBITaxon_9606 | HeLa | EFO | EFO_0001185 | M-018884-00 | D-018884-01;D-018884-02;D-018884-03;D-018884-04 | LOC51252 | NM_016490 | 31,745,175 | null | 51252 | LOC51252 | NM_016490 | 4 | null | NCBI36.3, RefSeq release 27, Jan 2008 | null | null | Alexa 488:tubulin;Hoechst:DNA;Tritc:actin | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | |
HT48_C10 | HT48 | 58 | C10 | Homo sapiens | NCBITaxon | NCBITaxon_9606 | HeLa | EFO | EFO_0001185 | M-016560-00 | D-016560-01;D-016560-02;D-016560-03;D-016560-04 | MGC33926 | NM_152390 | 22,748,834 | null | 130733 | TMEM178 | NM_152390 | 4 | null | NCBI36.3, RefSeq release 27, Jan 2008 | null | null | Alexa 488:tubulin;Hoechst:DNA;Tritc:actin | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | |
HT19_D17 | HT19 | 89 | D17 | Homo sapiens | NCBITaxon | NCBITaxon_9606 | HeLa | EFO | EFO_0001185 | M-016631-01 | D-016631-01;D-016631-04;D-016631-05;D-016631-06 | CTXL | NM_014312 | 34,147,668 | null | 23584 | VSIG2 | NM_014312 | 4 | null | NCBI36.3, RefSeq release 27, Jan 2008 | null | null | Alexa 488:tubulin;Hoechst:DNA;Tritc:actin | 0 | 0 | 0 | 0 | 0 | 0 | 0.767 | 0 | 0 | 0 | 0 | 0 | 0 | yes | multiple reagents | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | other phenotype | |
HT51_K7 | HT51 | 247 | K7 | Homo sapiens | NCBITaxon | NCBITaxon_9606 | HeLa | EFO | EFO_0001185 | M-018510-00 | D-018510-01;D-018510-02;D-018510-03;D-018510-04 | FLJ35838 | NM_173532 | 27,734,936 | null | 163479 | FNDC7 | NM_173532 | 4 | null | NCBI36.3, RefSeq release 27, Jan 2008 | null | null | Alexa 488:tubulin;Hoechst:DNA;Tritc:actin | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | |
HT18_M19 | HT18 | 307 | M19 | Homo sapiens | NCBITaxon | NCBITaxon_9606 | HeLa | EFO | EFO_0001185 | M-010562-01 | D-010562-01;D-010562-02;D-010562-03;D-010562-05 | NBR2 | NM_005821 | 14,141,183 | null | null | null | NR_003108;NM_001039083+NM_001103154 | 4;1+0 | siRNA matched to more than one gene (10230;641522,NBR2;ARL17) | NCBI36.3, RefSeq release 27, Jan 2008 | null | null | Alexa 488:tubulin;Hoechst:DNA;Tritc:actin | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | |
HT08_F12 | HT08 | 132 | F12 | Homo sapiens | NCBITaxon | NCBITaxon_9606 | HeLa | EFO | EFO_0001185 | M-019249-00 | D-019249-01;D-019249-02;D-019249-03;D-019249-04 | ERP70 | NM_004911 | 33,469,983 | null | 9601 | PDIA4 | NM_004911 | 4 | null | NCBI36.3, RefSeq release 27, Jan 2008 | null | null | Alexa 488:tubulin;Hoechst:DNA;Tritc:actin | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | |
HT58_N22 | HT58 | 334 | N22 | Homo sapiens | NCBITaxon | NCBITaxon_9606 | HeLa | EFO | EFO_0001185 | M-024936-00 | D-024936-01;D-024936-02;D-024936-03;D-024936-04 | LOC347541 | XM_293407 | 41,151,604 | null | null | null | XM_001715155;XM_942614+XM_293407 | 4;4+4 | siRNA matched to more than one gene (MAGEB5;347541,MAGEB5;MAGEB5) | NCBI36.3, RefSeq release 27, Jan 2008 | null | null | Alexa 488:tubulin;Hoechst:DNA;Tritc:actin | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | |
HT48_J5 | HT48 | 221 | J5 | Homo sapiens | NCBITaxon | NCBITaxon_9606 | HeLa | EFO | EFO_0001185 | M-015349-00 | D-015349-01;D-015349-02;D-015349-03;D-015349-04 | EMID2 | NM_133457 | 19,263,346 | null | 136227 | EMID2 | NM_133457 | 3 | null | NCBI36.3, RefSeq release 27, Jan 2008 | null | null | Alexa 488:tubulin;Hoechst:DNA;Tritc:actin | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | |
HT04_E24 | HT04 | 120 | E24 | Homo sapiens | NCBITaxon | NCBITaxon_9606 | HeLa | EFO | EFO_0001185 | M-003250-02 | D-003250-06;D-003250-07;D-003250-08;D-003250-09 | CENPB | NM_001810 | 26,105,977 | null | 1059 | CENPB | NM_001810 | 4 | null | NCBI36.3, RefSeq release 27, Jan 2008 | null | null | Alexa 488:tubulin;Hoechst:DNA;Tritc:actin | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | |
HT62_F8 | HT62 | 128 | F8 | Homo sapiens | NCBITaxon | NCBITaxon_9606 | HeLa | EFO | EFO_0001185 | M-029720-00 | D-029720-01;D-029720-02;D-029720-03;D-029720-04 | LOC389936 | XM_372303 | 41,149,263 | null | LOC389936 | LOC389936 | XR_041252+XR_041251+XR_041250 | 4+4+4 | null | NCBI36.3, RefSeq release 27, Jan 2008 | null | null | Alexa 488:tubulin;Hoechst:DNA;Tritc:actin | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | yes | multiple reagents | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | other phenotype | |
HT65_A15 | HT65 | 15 | A15 | Homo sapiens | NCBITaxon | NCBITaxon_9606 | HeLa | EFO | EFO_0001185 | M-031558-00 | D-031558-01;D-031558-02;D-031558-03;D-031558-04 | LOC401605 | XM_377027 | 42,662,582 | null | LOC643310 | LOC643310 | XR_038903+XR_038619+XR_038742 | 2+2+2 | null | NCBI36.3, RefSeq release 27, Jan 2008 | null | null | Alexa 488:tubulin;Hoechst:DNA;Tritc:actin | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | |
HT08_J19 | HT08 | 235 | J19 | Homo sapiens | NCBITaxon | NCBITaxon_9606 | HeLa | EFO | EFO_0001185 | M-005834-00 | D-005834-01;D-005834-02;D-005834-03;D-005834-04 | CTRL | NM_001907 | 4,503,136 | null | 1506 | CTRL | NM_001907 | 4 | null | NCBI36.3, RefSeq release 27, Jan 2008 | null | null | Alexa 488:tubulin;Hoechst:DNA;Tritc:actin | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | |
HT39_M12 | HT39 | 300 | M12 | Homo sapiens | NCBITaxon | NCBITaxon_9606 | HeLa | EFO | EFO_0001185 | M-010046-00 | D-010046-01;D-010046-02;D-010046-03;D-010046-04 | NEUROD4 | NM_021191 | 10,863,998 | null | 58158 | NEUROD4 | NM_021191 | 4 | null | NCBI36.3, RefSeq release 27, Jan 2008 | null | null | Alexa 488:tubulin;Hoechst:DNA;Tritc:actin | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | |
HT26_A8 | HT26 | 8 | A8 | Homo sapiens | NCBITaxon | NCBITaxon_9606 | HeLa | EFO | EFO_0001185 | M-011142-00 | D-011142-01;D-011142-02;D-011142-03;D-011142-04 | RPL18 | NM_000979 | 15,431,298 | null | null | null | NM_000979;XR_019588;XR_042366+XR_042413 | 4;1;1+1 | siRNA matched to more than one gene (6141;441775;LOC441775,RPL18;LOC441775;LOC441775) | NCBI36.3, RefSeq release 27, Jan 2008 | null | null | Alexa 488:tubulin;Hoechst:DNA;Tritc:actin | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | |
HT04_M11 | HT04 | 299 | M11 | Homo sapiens | NCBITaxon | NCBITaxon_9606 | HeLa | EFO | EFO_0001185 | M-004396-00 | D-004396-01;D-004396-02;D-004396-03;D-004396-04 | CARD12 | NM_021209 | 20,127,597 | null | 58484 | NLRC4 | NM_021209 | 4 | null | NCBI36.3, RefSeq release 27, Jan 2008 | null | null | Alexa 488:tubulin;Hoechst:DNA;Tritc:actin | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | |
HT62_N21 | HT62 | 333 | N21 | Homo sapiens | NCBITaxon | NCBITaxon_9606 | HeLa | EFO | EFO_0001185 | M-029660-00 | D-029660-01;D-029660-02;D-029660-03;D-029660-04 | LOC387712 | XM_370577 | 42,659,388 | null | null | null | null | null | null | NCBI36.3, RefSeq release 27, Jan 2008 | null | null | Alexa 488:tubulin;Hoechst:DNA;Tritc:actin | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | |
HT36_H10 | HT36 | 178 | H10 | Homo sapiens | NCBITaxon | NCBITaxon_9606 | HeLa | EFO | EFO_0001185 | M-020869-00 | D-020869-01;D-020869-02;D-020869-03;D-020869-04 | FLJ10853 | NM_018246 | 8,922,717 | null | 55246 | CCDC25 | NM_018246 | 4 | null | NCBI36.3, RefSeq release 27, Jan 2008 | null | null | Alexa 488:tubulin;Hoechst:DNA;Tritc:actin | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | |
HT31_M13 | HT31 | 301 | M13 | Homo sapiens | NCBITaxon | NCBITaxon_9606 | HeLa | EFO | EFO_0001185 | M-008076-00 | D-008076-01;D-008076-02;D-008076-03;D-008076-04 | SARM1 | NM_015077 | 14,149,666 | null | 23098 | SARM1 | NM_015077 | 4 | null | NCBI36.3, RefSeq release 27, Jan 2008 | null | null | Alexa 488:tubulin;Hoechst:DNA;Tritc:actin | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | |
HT62_P12 | HT62 | 372 | P12 | Homo sapiens | NCBITaxon | NCBITaxon_9606 | HeLa | EFO | EFO_0001185 | M-029865-00 | D-029865-01;D-029865-02;D-029865-03;D-029865-04 | LOC387784 | XM_373506 | 41,200,365 | null | null | null | XM_001129354;XM_001716717 | 1;1 | siRNA matched to more than one gene (729113;LOC729113,LOC729113;LOC729113) | NCBI36.3, RefSeq release 27, Jan 2008 | null | null | Alexa 488:tubulin;Hoechst:DNA;Tritc:actin | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | |
HT51_O23 | HT51 | 359 | O23 | Homo sapiens | NCBITaxon | NCBITaxon_9606 | HeLa | EFO | EFO_0001185 | M-017185-00 | D-017185-01;D-017185-02;D-017185-03;D-017185-04 | MGC35154 | NM_152614 | 22,749,256 | null | 165100 | C2orf57 | NM_152614 | 4 | null | NCBI36.3, RefSeq release 27, Jan 2008 | null | null | Alexa 488:tubulin;Hoechst:DNA;Tritc:actin | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | |
HT62_E8 | HT62 | 104 | E8 | Homo sapiens | NCBITaxon | NCBITaxon_9606 | HeLa | EFO | EFO_0001185 | M-029364-00 | D-029364-01;D-029364-02;D-029364-03;D-029364-04 | LOC392262 | XM_373263 | 41,148,687 | null | null | null | null | null | null | NCBI36.3, RefSeq release 27, Jan 2008 | null | null | Alexa 488:tubulin;Hoechst:DNA;Tritc:actin | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | |
HT48_P22 | HT48 | 382 | P22 | Homo sapiens | NCBITaxon | NCBITaxon_9606 | HeLa | EFO | EFO_0001185 | M-013695-00 | D-013695-01;D-013695-02;D-013695-03;D-013695-04 | WFDC6 | NM_080827 | 22,779,935 | null | 140870 | WFDC6 | NM_080827 | 4 | null | NCBI36.3, RefSeq release 27, Jan 2008 | null | null | Alexa 488:tubulin;Hoechst:DNA;Tritc:actin | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | |
HT58_D12 | HT58 | 84 | D12 | Homo sapiens | NCBITaxon | NCBITaxon_9606 | HeLa | EFO | EFO_0001185 | M-024531-00 | D-024531-01;D-024531-02;D-024531-03;D-024531-04 | LOC340120 | XM_293976 | 37,550,397 | null | 340120 | ANKRD34B | NM_001004441 | 4 | null | NCBI36.3, RefSeq release 27, Jan 2008 | null | null | Alexa 488:tubulin;Hoechst:DNA;Tritc:actin | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | |
HT65_G17 | HT65 | 161 | G17 | Homo sapiens | NCBITaxon | NCBITaxon_9606 | HeLa | EFO | EFO_0001185 | M-031626-00 | D-031626-01;D-031626-02;D-031626-03;D-031626-04 | LOC392539 | XM_373370 | 41,151,649 | null | null | null | null | null | null | NCBI36.3, RefSeq release 27, Jan 2008 | null | null | Alexa 488:tubulin;Hoechst:DNA;Tritc:actin | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | |
HT65_O22 | HT65 | 358 | O22 | Homo sapiens | NCBITaxon | NCBITaxon_9606 | HeLa | EFO | EFO_0001185 | M-032379-00 | D-032379-01;D-032379-02;D-032379-03;D-032379-04 | OR4F4 | NM_001004195 | 57,528,065 | null | null | null | NM_001004195;NM_001005240;NM_001005484 | 4;3;2 | siRNA matched to more than one gene (26682;81099;79501,OR4F4;OR4F17;OR4F5) | NCBI36.3, RefSeq release 27, Jan 2008 | null | null | Alexa 488:tubulin;Hoechst:DNA;Tritc:actin | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | |
HT65_N20 | HT65 | 332 | N20 | Homo sapiens | NCBITaxon | NCBITaxon_9606 | HeLa | EFO | EFO_0001185 | M-032532-00 | D-032532-01;D-032532-02;D-032532-03;D-032532-04 | OR6B3 | NM_173351 | 50,051,734 | null | null | null | NM_173351;NM_001005853 | 4;3 | siRNA matched to more than one gene (150681;389090,OR6B3;OR6B2) | NCBI36.3, RefSeq release 27, Jan 2008 | null | null | Alexa 488:tubulin;Hoechst:DNA;Tritc:actin | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | |
HT18_O8 | HT18 | 344 | O8 | Homo sapiens | NCBITaxon | NCBITaxon_9606 | HeLa | EFO | EFO_0001185 | M-008113-01 | D-008113-02;D-008113-03;D-008113-04;D-008113-05 | ADH4 | NM_000670 | 11,496,889 | null | 127 | ADH4 | NM_000670 | 4 | null | NCBI36.3, RefSeq release 27, Jan 2008 | null | null | Alexa 488:tubulin;Hoechst:DNA;Tritc:actin | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | |
HT65_G14 | HT65 | 158 | G14 | Homo sapiens | NCBITaxon | NCBITaxon_9606 | HeLa | EFO | EFO_0001185 | M-032334-00 | D-032334-01;D-032334-02;D-032334-03;D-032334-04 | MGC21382 | NM_001001850 | 49,258,195 | null | 415117 | STX19 | NM_001001850 | 4 | null | NCBI36.3, RefSeq release 27, Jan 2008 | null | null | Alexa 488:tubulin;Hoechst:DNA;Tritc:actin | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | |
HT04_N5 | HT04 | 317 | N5 | Homo sapiens | NCBITaxon | NCBITaxon_9606 | HeLa | EFO | EFO_0001185 | M-017257-01 | D-017257-01;D-017257-03;D-017257-04;D-017257-05 | CD81 | NM_004356 | 21,237,760 | null | 975 | CD81 | NM_004356 | 4 | null | NCBI36.3, RefSeq release 27, Jan 2008 | null | null | Alexa 488:tubulin;Hoechst:DNA;Tritc:actin | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | |
HT65_G4 | HT65 | 148 | G4 | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | empty well | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | |
HT36_M20 | HT36 | 308 | M20 | Homo sapiens | NCBITaxon | NCBITaxon_9606 | HeLa | EFO | EFO_0001185 | M-021099-00 | D-021099-01;D-021099-02;D-021099-03;D-021099-04 | FLJ10199 | NM_018022 | 8,922,276 | null | 55092 | TMEM51 | NM_018022 | 4 | null | NCBI36.3, RefSeq release 27, Jan 2008 | null | null | Alexa 488:tubulin;Hoechst:DNA;Tritc:actin | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | |
HT11_D10 | HT11 | 82 | D10 | Homo sapiens | NCBITaxon | NCBITaxon_9606 | HeLa | EFO | EFO_0001185 | M-010325-01 | D-010325-01;D-010325-02;D-010325-03;D-010325-05 | TUBB | NM_178014 | 34,222,261 | null | null | null | XR_018705;NM_178014;XR_016140;XR_041042 | 4;4;4;4 | siRNA matched to more than one gene (648765;203068;92755;LOC92755,LOC92755;TUBB;LOC92755;LOC92755) | NCBI36.3, RefSeq release 27, Jan 2008 | null | null | Alexa 488:tubulin;Hoechst:DNA;Tritc:actin | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | |
HT34_O9 | HT34 | 345 | O9 | Homo sapiens | NCBITaxon | NCBITaxon_9606 | HeLa | EFO | EFO_0001185 | M-020955-00 | D-020955-01;D-020955-02;D-020955-03;D-020955-04 | LOC51059 | NM_015912 | 24,475,966 | null | 51059 | FAM135B | NM_015912 | 4 | null | NCBI36.3, RefSeq release 27, Jan 2008 | null | null | Alexa 488:tubulin;Hoechst:DNA;Tritc:actin | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | |
HT58_D15 | HT58 | 87 | D15 | Homo sapiens | NCBITaxon | NCBITaxon_9606 | HeLa | EFO | EFO_0001185 | M-024055-00 | D-024055-01;D-024055-02;D-024055-03;D-024055-04 | LOC345051 | XM_293680 | 37,540,053 | null | LOC345051 | LOC345051 | XR_040589+XR_040587+XR_040588 | 4+4+4 | null | NCBI36.3, RefSeq release 27, Jan 2008 | null | null | Alexa 488:tubulin;Hoechst:DNA;Tritc:actin | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | |
HT20_E23 | HT20 | 119 | E23 | Homo sapiens | NCBITaxon | NCBITaxon_9606 | HeLa | EFO | EFO_0001185 | M-020676-00 | D-020676-01;D-020676-02;D-020676-03;D-020676-04 | FLJ20184 | NM_017700 | 8,923,172 | null | 54848 | FLJ20184 | NM_017700 | 4 | null | NCBI36.3, RefSeq release 27, Jan 2008 | null | null | Alexa 488:tubulin;Hoechst:DNA;Tritc:actin | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | |
HT36_L5 | HT36 | 269 | L5 | Homo sapiens | NCBITaxon | NCBITaxon_9606 | HeLa | EFO | EFO_0001185 | M-020768-00 | D-020768-01;D-020768-02;D-020768-03;D-020768-04 | C6ORF139 | NM_018132 | 46,255,020 | null | 55166 | CENPQ | NM_018132 | 4 | null | NCBI36.3, RefSeq release 27, Jan 2008 | null | null | Alexa 488:tubulin;Hoechst:DNA;Tritc:actin | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | |
HT13_G7 | HT13 | 151 | G7 | Homo sapiens | NCBITaxon | NCBITaxon_9606 | HeLa | EFO | EFO_0001185 | M-009595-01 | D-009595-01;D-009595-02;D-009595-03;D-009595-05 | KIF3B | NM_004798 | 31,742,486 | null | 9371 | KIF3B | NM_004798 | 4 | null | NCBI36.3, RefSeq release 27, Jan 2008 | null | null | Alexa 488:tubulin;Hoechst:DNA;Tritc:actin | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | |
HT33_K12 | HT33 | 252 | K12 | Homo sapiens | NCBITaxon | NCBITaxon_9606 | HeLa | EFO | EFO_0001185 | M-013715-00 | D-013715-01;D-013715-02;D-013715-03;D-013715-04 | NP220 | NM_014497 | 21,626,467 | null | 27332 | ZNF638 | NM_001014972+NM_014497 | 4+4 | null | NCBI36.3, RefSeq release 27, Jan 2008 | null | null | Alexa 488:tubulin;Hoechst:DNA;Tritc:actin | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | yes | multiple reagents | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | proliferating cells | proliferating cells | CMPO_0000241 | null | |
HT20_N24 | HT20 | 336 | N24 | Homo sapiens | NCBITaxon | NCBITaxon_9606 | HeLa | EFO | EFO_0001185 | M-014332-00 | D-014332-01;D-014332-02;D-014332-03;D-014332-04 | LENG1 | NM_024316 | 24,308,288 | null | 79165 | LENG1 | NM_024316 | 4 | null | NCBI36.3, RefSeq release 27, Jan 2008 | null | null | Alexa 488:tubulin;Hoechst:DNA;Tritc:actin | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | |
HT51_L17 | HT51 | 281 | L17 | Homo sapiens | NCBITaxon | NCBITaxon_9606 | HeLa | EFO | EFO_0001185 | M-018776-00 | D-018776-01;D-018776-02;D-018776-03;D-018776-04 | DCP1B | NM_152640 | 40,548,402 | null | 196513 | DCP1B | NM_152640 | 4 | null | NCBI36.3, RefSeq release 27, Jan 2008 | null | null | Alexa 488:tubulin;Hoechst:DNA;Tritc:actin | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | |
HT58_M18 | HT58 | 306 | M18 | Homo sapiens | NCBITaxon | NCBITaxon_9606 | HeLa | EFO | EFO_0001185 | M-023933-00 | D-023933-01;D-023933-02;D-023933-03;D-023933-04 | LOC90024 | XM_028217 | 42,656,936 | null | null | null | XM_944210+XM_028217;XM_001717455 | 4+4;4 | siRNA matched to more than one gene (90024;FLJ20021,FLJ20021;FLJ20021) | NCBI36.3, RefSeq release 27, Jan 2008 | null | null | Alexa 488:tubulin;Hoechst:DNA;Tritc:actin | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | |
HT11_I13 | HT11 | 205 | I13 | Homo sapiens | NCBITaxon | NCBITaxon_9606 | HeLa | EFO | EFO_0001185 | M-009429-01 | D-009429-01;D-009429-02;D-009429-03;D-009429-05 | MCCC1 | NM_020166 | 13,518,227 | null | 56922 | MCCC1 | NM_020166 | 4 | null | NCBI36.3, RefSeq release 27, Jan 2008 | null | null | Alexa 488:tubulin;Hoechst:DNA;Tritc:actin | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | |
HT15_F18 | HT15 | 138 | F18 | Homo sapiens | NCBITaxon | NCBITaxon_9606 | HeLa | EFO | EFO_0001185 | M-021258-00 | D-021258-01;D-021258-02;D-021258-03;D-021258-04 | KAB | NM_014812 | 7,662,141 | null | null | null | NM_014812+NM_001042405+NM_001042404;XR_042148+XR_042173;XM_001713866 | 4+3+3;3+2;2 | siRNA matched to more than one gene (9859;CEP170L;LOC100133718,CEP170;CEP170L;LOC100133718) | NCBI36.3, RefSeq release 27, Jan 2008 | null | null | Alexa 488:tubulin;Hoechst:DNA;Tritc:actin | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | |
HT65_L21 | HT65 | 285 | L21 | Homo sapiens | NCBITaxon | NCBITaxon_9606 | HeLa | EFO | EFO_0001185 | M-032440-00 | D-032440-01;D-032440-02;D-032440-03;D-032440-04 | OR10A7 | NM_001005280 | 52,627,159 | null | 121364 | OR10A7 | NM_001005280 | 4 | null | NCBI36.3, RefSeq release 27, Jan 2008 | null | null | Alexa 488:tubulin;Hoechst:DNA;Tritc:actin | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | |
HT34_E4 | HT34 | 100 | E4 | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | empty well | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | |
HT04_E20 | HT04 | 116 | E20 | Homo sapiens | NCBITaxon | NCBITaxon_9606 | HeLa | EFO | EFO_0001185 | M-011608-00 | D-011608-01;D-011608-02;D-011608-03;D-011608-04 | CEBPG | NM_001806 | 34,452,718 | null | 1054 | CEBPG | NM_001806 | 4 | null | NCBI36.3, RefSeq release 27, Jan 2008 | null | null | Alexa 488:tubulin;Hoechst:DNA;Tritc:actin | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | |
HT31_O20 | HT31 | 356 | O20 | Homo sapiens | NCBITaxon | NCBITaxon_9606 | HeLa | EFO | EFO_0001185 | M-022715-00 | D-022715-01;D-022715-02;D-022715-03;D-022715-04 | SEC15L2 | XM_039570 | 42,656,337 | null | 23233 | EXOC6B | NM_015189 | 4 | null | NCBI36.3, RefSeq release 27, Jan 2008 | null | null | Alexa 488:tubulin;Hoechst:DNA;Tritc:actin | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | |
HT13_F5 | HT13 | 125 | F5 | Homo sapiens | NCBITaxon | NCBITaxon_9606 | HeLa | EFO | EFO_0001185 | M-007916-00 | D-007916-01;D-007916-02;D-007916-03;D-007916-04 | GFRA4 | NM_022139 | 22,091,446 | null | 64096 | GFRA4 | NM_145762+NM_022139 | 4+4 | null | NCBI36.3, RefSeq release 27, Jan 2008 | null | null | Alexa 488:tubulin;Hoechst:DNA;Tritc:actin | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | |
HT11_B15 | HT11 | 39 | B15 | Homo sapiens | NCBITaxon | NCBITaxon_9606 | HeLa | EFO | EFO_0001185 | M-006014-02 | D-006014-03;D-006014-04;D-006014-05;D-006014-06 | PRSS25 | NM_013247 | 21,614,536 | null | 27429 | HTRA2 | NM_145074+NM_013247 | 4+4 | null | NCBI36.3, RefSeq release 27, Jan 2008 | null | null | Alexa 488:tubulin;Hoechst:DNA;Tritc:actin | 0 | 0.792 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | yes | multiple reagents | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | other phenotype | |
HT31_H16 | HT31 | 184 | H16 | Homo sapiens | NCBITaxon | NCBITaxon_9606 | HeLa | EFO | EFO_0001185 | M-021474-00 | D-021474-01;D-021474-02;D-021474-03;D-021474-04 | AP4E1 | NM_007347 | 37,675,282 | null | 23431 | AP4E1 | NM_007347 | 4 | null | NCBI36.3, RefSeq release 27, Jan 2008 | null | null | Alexa 488:tubulin;Hoechst:DNA;Tritc:actin | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | |
HT33_D13 | HT33 | 85 | D13 | Homo sapiens | NCBITaxon | NCBITaxon_9606 | HeLa | EFO | EFO_0001185 | M-020573-00 | D-020573-01;D-020573-02;D-020573-03;D-020573-04 | BZW2 | NM_014038 | 7,661,743 | null | 28969 | BZW2 | NM_014038 | 4 | null | NCBI36.3, RefSeq release 27, Jan 2008 | null | null | Alexa 488:tubulin;Hoechst:DNA;Tritc:actin | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null | null |
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Clustering phenotype populations by genome-wide RNAi and multiparametric imaging
52224 images of a high-content RNAi knockdown screening.
Author one-liner: "To cluster genes and predict function on a genome-wide scale, we measured the effects of 22 839 siRNA-mediated knockdowns on HeLa cells. Each siRNA effect was summarized by a phenotypic profile."
Dataset Details
Organism: human (Homo sapiens) Cell type: HeLa Imaging method: Fluorescence microscopy
Study metadata: metadata/S-EPMC2913390.json - authors and IDs (BioStudies extract); metadata/msb201025-s1.pdf - Supplementary information (incl. variable definitions); metadata/msb201025-s1.xls - biological process annotation for some wells.
Dataset Description
Genetic screens for phenotypic similarity have made key contributions to associating genes with biological processes. With RNA interference (RNAi), highly parallel phenotyping of loss-of-function effects in cells has become feasible. One of the current challenges however is the computational categorization of visual phenotypes and the prediction of biological function and processes. In this study, we describe a combined computational and experimental approach to discover novel gene functions and explore functional relationships. We performed a genome-wide RNAi screen in human cells and used quantitative descriptors derived from high-throughput imaging to generate multiparametric phenotypic profiles. We show that profiles predicted functions of genes by phenotypic similarity. Specifically, we examined several candidates including the largely uncharacterized gene DONSON, which shared phenotype similarity with known factors of DNA damage response (DDR) and genomic integrity. Experimental evidence supports that DONSON is a novel centrosomal protein required for DDR signalling and genomic integrity. Multiparametric phenotyping by automated imaging and computational annotation is a powerful method for functional discovery and mapping the landscape of phenotypic responses to cellular perturbations.
- Curated by: University of Dundee
- Funded by: IDR: BBSRC (Ref: BB/M018423/1), 688945 (Euro-BioImaging Prep Phase II), 653493 (Global BioImaging Project), 654248 (CORBEL), Wellcome Trust (Ref: 212962/Z/18/Z)
- Shared by: IDR
- License: CC BY-NC-ND 4.0
Dataset Sources
- Repository: IDR, Bioimage Archive,
- Paper [optional]: Clustering phenotype populations by genome‐wide RNAi and multiparametric imaging
Uses
Machine Learning pipelines for Bioimaging, drug screening data.
Dataset Structure
Dataset is structured in High-Content-Screening structure of OMERO. Images are 16-bit PNG converted from OME-Zarr. NB!: be aware of automatic 8-bit conversion by image tools, e.g. OpenCV!
Metadata is provided in the tabular format (CSV) as well as JSON-LD objects.
Dataset Creation
Curation Rationale
Making Bioimaging experiment data available for AI models.
Source Data
More information on experiments
Data Collection and Processing
Who are the source data producers?
Florian Fuchs, Gregoire Pau, Dominique Kranz, Oleg Sklyar, Christoph Budjan, Sandra Steinbrink, Thomas Horn, Angelika Pedal, Wolfgang Huber m.boutros@dkfz.de, and Michael Boutros
Annotations [optional]
Annotation process
https://idr.openmicroscopy.org/about/screens.html
Who are the annotators?
IDR staff and original scientists
Personal and Sensitive Information
No human PII / PHI has been noticed in the samples.
Bias, Risks, and Limitations
Experiments could have contained errors / artifacts. Please refer to the original paper for more details.
Recommendations
NB!: be aware of automatic 8-bit conversion by image tools, e.g. OpenCV!
Original Zarr files contain a better resolution / additional lazy loading capabilities.
Citation
BibTeX:
@article{fuchs2010clustering, title={Clustering phenotype populations by genome-wide RNAi and multiparametric imaging}, author={Fuchs, Florian and Pau, Gregoire and Kranz, Dominique and Sklyar, Oleg and Budjan, Christoph and Steinbrink, Sandra and Horn, Thomas and Pedal, Angelika and Huber, Wolfgang and Boutros, Michael}, journal={Molecular systems biology}, volume={6}, number={1}, pages={370}, year={2010}, publisher={John Wiley & Sons, Ltd Chichester, UK} }
APA:
Fuchs, F., Pau, G., Kranz, D., Sklyar, O., Budjan, C., Steinbrink, S., ... & Boutros, M. (2010). Clustering phenotype populations by genome‐wide RNAi and multiparametric imaging. Molecular systems biology, 6(1), 370.
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